Image taken on a Zeiss 710 Confocal microscope at the University of Dundee.
Image taken on a Zeiss 710 Confocal microscope at the University of Dundee.
Image taken on a Zeiss 710 Confocal microscope at the University of Dundee.
Image taken on a Zeiss 710 Confocal microscope at the University of Dundee, but then put through a filter.
The last images are put though the very professional “Glowing edges” filter found in Microsoft Office Powerpoint.
Hi Val,
Looks really cool. Can you tell a little about what we are seeing and in what context?
Thanks!
(p.s. : Check your mail )
Hi Thomas,
What you see here are MDCK cells (top image) and cysts (middle 2 images). MDCK cells (Madine-Darby Canine Kidney cells) are an immortal cell-line of dog kidney cells. They’re epithelial cells and form round hollow cysts in 3D culture conditions. I’m using them while I establish techniques (because they’re an immortal cell-line, easier to work with, they’re a good model for gut organoids which are my final goal).
I have stained for the nucleus (Hoescht, blue), actin filaments (green) and microtubules (red).
Perhaps this clears things up a bit?